A simple, efficient, precise and isocratic reverse phase-high performance liquid chromatographic method was developed and validated as per ICH guidelines for the determination of L-carnitine and L-tartrate in bulk and pharmaceutical dosage form. The chromatographic separation was achieved by using prontosil, C18, 5µ, (4.6 x 250) column at ambient temperature (30°c). Mobile phase comprises of orthophosphoric acid: water (1:999 v/v) and was carried at a flow rate of 1.0 ml/min with an injection volume of 20 µl, while the detection was monitored at a wavelength of 210 nm and the run time is 8 min. The developed method gave excellent linear response for both L-carnitine and L-tartrate were in the range of 100-600 µg/ml. The accuracy of the developed method was explained by percent recovery studies ranged from 99.4 to 99.5% for L-carnitine and 98.95 to 99.53 for L-tartrate. The retention time for L-carnitine in an average was observed as 2.59 and for L-tartrate it was 3.6 minutes. The proposed method was applied successfully for the analysis of drug in pure and in its pharmaceutical dosage forms and validated according to ICH guidelines.
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